67.6 Folate:Active B12

67.6.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

67.6.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.93 (2.45, 3.41) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.05
f39_gdm_newGDM -0.24 (-0.55, 0.07) 0.13
StudySTOP -0.23 (-0.40, -0.06) 0.008
GA_samp 0.13 (0.10, 0.16) <0.0001
folate_Active.B12:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.60
f39_gdm_newGDM:StudySTOP 0.21 (-0.10, 0.52) 0.17
folate_Active.B12:StudySTOP 0.00 (0.00, 0.00) 0.03
HPL
Var Effect P
(Intercept) 0.67 (0.42, 0.93) <0.0001
folate_Active.B12 0.00 (-0.00, 0.00) 0.65
f39_gdm_newGDM -0.13 (-0.28, 0.02) 0.09
StudySTOP 0.16 (0.07, 0.25) 0.0005
GA_samp 0.29 (0.27, 0.30) <0.0001
folate_Active.B12:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.53
f39_gdm_newGDM:StudySTOP 0.11 (-0.04, 0.26) 0.15
folate_Active.B12:StudySTOP -0.00 (-0.00, -0.00) 0.02
GH2
Var Effect P
(Intercept) -2.68 (-3.03, -2.34) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.13
f39_gdm_newGDM -0.17 (-0.38, 0.05) 0.13
StudySTOP -0.07 (-0.19, 0.06) 0.28
GA_samp 0.27 (0.25, 0.29) <0.0001
folate_Active.B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.89
f39_gdm_newGDM:StudySTOP 0.18 (-0.04, 0.40) 0.11
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.29
Insulin
Var Effect P
(Intercept) 2.66 (2.05, 3.26) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.66
f39_gdm_newGDM 0.97 (0.43, 1.51) 0.0004
StudySTOP -0.31 (-0.54, -0.07) 0.01
GA_samp 0.01 (-0.03, 0.04) 0.74
folate_Active.B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.69
f39_gdm_newGDM:StudySTOP -0.35 (-0.96, 0.25) 0.25
folate_Active.B12:StudySTOP -0.00 (-0.00, 0.00) 0.89
folate_Active.B12:f39_gdm_newGDM:StudySTOP -0.00 (-0.00, 0.00) 0.68
HOMA.IR
Var Effect P
(Intercept) 1.36 (0.71, 2.00) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.65
f39_gdm_newGDM 1.04 (0.42, 1.66) 0.001
StudySTOP -0.47 (-0.73, -0.22) 0.0002
GA_samp -0.00 (-0.04, 0.04) 0.94
folate_Active.B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.65
f39_gdm_newGDM:StudySTOP -0.37 (-1.06, 0.32) 0.29
folate_Active.B12:StudySTOP -0.00 (-0.00, 0.00) 0.89
folate_Active.B12:f39_gdm_newGDM:StudySTOP -0.00 (-0.00, 0.00) 0.60
GLUC3_mmol.L
Var Effect P
(Intercept) 1.81 (1.68, 1.95) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.16
f39_gdm_newGDM 0.17 (-0.01, 0.36) 0.07
StudySTOP -0.16 (-0.20, -0.11) <0.0001
GA_samp -0.01 (-0.02, 0.00) 0.10
folate_Active.B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.08
f39_gdm_newGDM:StudySTOP -0.13 (-0.31, 0.05) 0.15
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.79

67.6.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:Active B12 ratio and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 103.67295 8.607879 98.74735 87.92535 122.2410
STOP non-GDM 91.59491 7.337537 85.97214 78.11050 107.4072
SCOPE GDM 84.74948 13.335572 646.80044 62.22243 115.4322
STOP GDM 92.62091 9.551892 215.33962 75.58393 113.4981
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 93.68393 2.807221 204.3840 88.30943 99.38552
STOP non-GDM 103.51862 2.950107 171.1750 97.85610 109.50880
SCOPE GDM 84.16705 5.935862 553.3811 73.27903 96.67284
STOP GDM 104.09156 4.692758 537.1804 95.26956 113.73048
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.424603 0.0833960 340.9341 2.265994 2.594315
STOP non-GDM 2.353344 0.0753146 311.0320 2.209722 2.506299
SCOPE GDM 2.035655 0.2011072 715.8881 1.676755 2.471376
STOP GDM 2.364709 0.1320586 607.8943 2.119079 2.638812
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 15.14587 0.9305930 Inf 13.427488 17.08417
STOP non-GDM 11.03849 0.5683307 Inf 9.978941 12.21054
SCOPE GDM 38.40424 7.6683352 Inf 25.966668 56.79919
STOP GDM 18.65766 1.9151286 Inf 15.257560 22.81547
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.705064 0.2450049 Inf 3.254680 4.217773
STOP non-GDM 2.283943 0.1252114 Inf 2.051258 2.543022
SCOPE GDM 10.010554 2.3228700 Inf 6.352483 15.775121
STOP GDM 3.959116 0.4342997 Inf 3.193190 4.908759
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.505121 0.0619100 Inf 5.385107 5.627809
STOP non-GDM 4.722670 0.0538362 Inf 4.618323 4.829374
SCOPE GDM 6.276927 0.5367242 Inf 5.308391 7.422174
STOP GDM 4.726920 0.0774446 Inf 4.577542 4.881171

67.6.4 Comparisons

67.6.4.1 Within Groups

Effect for every 500 unit increase in Folate:Active B12 ratio.
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 0.965 (0.931, 1.000) 0.05
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 1.025 (0.983, 1.070) 0.2
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.986 (0.907, 1.073) 0.7
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 1.048 (0.975, 1.126) 0.2
HPL folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 1.004 (0.986, 1.023) 0.6
HPL folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 0.970 (0.948, 0.993) 0.010
HPL folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 1.018 (0.974, 1.064) 0.4
HPL folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.983 (0.946, 1.022) 0.4
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 0.980 (0.956, 1.006) 0.1
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 1.002 (0.970, 1.036) 0.9
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.976 (0.917, 1.039) 0.4
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.998 (0.946, 1.053) 0.9
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 0.986 (0.927, 1.049) 0.7
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 0.981 (0.931, 1.033) 0.5
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.966 (0.889, 1.049) 0.4
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.931 (0.851, 1.019) 0.1
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 0.985 (0.921, 1.053) 0.7
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 0.979 (0.926, 1.034) 0.4
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.958 (0.870, 1.055) 0.4
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.913 (0.833, 1.001) 0.05
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE non-GDM 0.996 (0.989, 1.002) 0.2
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 STOP non-GDM 0.997 (0.985, 1.010) 0.7
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.973 (0.947, 1.000) 0.05
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.975 (0.955, 0.995) 0.01

67.6.4.2 Between Groups

Below are comparisons of the differences in the change in Folate:Active B12 ratio between groups. I.e. whether the change in Folate:Active B12 ratio are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.129008 0.0624241 1070.3772 1.0129309 1.258387 0.028 *
STOP/SCOPE (non-GDM) 1.062548 0.0293747 1070.3772 1.0064447 1.121778 0.028 *
STOP/SCOPE (GDM) 1.062548 0.0293747 1070.3772 1.0064447 1.121778 0.028 *
GDM/non-GDM (SCOPE) 1.021830 0.0416747 972.1293 0.9432344 1.106975 0.597 ns
GDM/non-GDM (STOP) 1.021830 0.0416747 972.1293 0.9432344 1.106975 0.597 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9332675 0.0273714 1027.9503 0.8810737 0.9885533 0.019 *
STOP/SCOPE (non-GDM) 0.9660577 0.0141665 1027.9503 0.9386552 0.9942602 0.019 *
STOP/SCOPE (GDM) 0.9660577 0.0141665 1027.9503 0.9386552 0.9942602 0.019 *
GDM/non-GDM (SCOPE) 1.0135354 0.0219233 891.5394 0.9714087 1.0574889 0.534 ns
GDM/non-GDM (STOP) 1.0135354 0.0219233 891.5394 0.9714087 1.0574889 0.534 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0448347 0.0434404 1080.3324 0.9629818 1.133645 0.292 ns
STOP/SCOPE (non-GDM) 1.0221715 0.0212491 1080.3324 0.9813163 1.064728 0.292 ns
STOP/SCOPE (GDM) 1.0221715 0.0212491 1080.3324 0.9813163 1.064728 0.292 ns
GDM/non-GDM (SCOPE) 0.9956797 0.0302511 977.0011 0.9380501 1.056850 0.887 ns
GDM/non-GDM (STOP) 0.9956797 0.0302511 977.0011 0.9380501 1.056850 0.887 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9587955 0.0717422 Inf 0.8280079 1.110242 0.574 ns
STOP/SCOPE (non-GDM) 0.9942499 0.0408609 Inf 0.9173046 1.077650 0.888 ns
STOP/SCOPE (GDM) 0.9643405 0.0603443 Inf 0.8530331 1.090172 0.562 ns
GDM/non-GDM (SCOPE) 0.9792031 0.0517149 Inf 0.8829132 1.085994 0.691 ns
GDM/non-GDM (STOP) 0.9497463 0.0504299 Inf 0.8558748 1.053913 0.332 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9471149 0.0769590 Inf 0.8076762 1.110627 0.504 ns
STOP/SCOPE (non-GDM) 0.9938934 0.0442180 Inf 0.9108989 1.084450 0.890 ns
STOP/SCOPE (GDM) 0.9529341 0.0648199 Inf 0.8339941 1.088837 0.478 ns
GDM/non-GDM (SCOPE) 0.9728165 0.0584730 Inf 0.8647047 1.094445 0.647 ns
GDM/non-GDM (STOP) 0.9327258 0.0510453 Inf 0.8378577 1.038336 0.203 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0038962 0.0144286 Inf 0.9760112 1.032578 0.787 ns
STOP/SCOPE (non-GDM) 1.0019462 0.0072003 Inf 0.9879328 1.016158 0.787 ns
STOP/SCOPE (GDM) 1.0019462 0.0072003 Inf 0.9879328 1.016158 0.787 ns
GDM/non-GDM (SCOPE) 0.9772807 0.0126769 Inf 0.9527476 1.002446 0.076 ns
GDM/non-GDM (STOP) 0.9772807 0.0126769 Inf 0.9527476 1.002446 0.076 ns

67.6.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

67.6.6 Plots